Modified on 10/26/2005 at 00:19
objectives
Our
project aims at the development of bioinformatic methods to identify
protein-protein interaction sites involving regulatory domains,
building blocks of modular proteins. These domains specifically
recognise small sequence motifs. We are developing new methods to
predict the binding specificities for each domain of interest using a
combination of structure prediction and in silico screening
of
peptide libraries. These predictions will be validated experimentally
on domains often involved in the DNA repair process (FHA, BRCT, Polo or
Tudor). Based on in
vitro assays and in
vivo yeast experiments, the
mutagenesis of the interaction site will provide unique tools to
dissect the importance of every interaction in the complex networks of
interactions.
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DESCRIPTION
