Supplementary Information associated with the manuscript :

Detection of a tandem BRCT in Xrs2 and Nbs1 with functional implications in the DNA damage response.

Emmanuelle Becker, Vincent Meyer, Hocine Madaoui, and Raphaël Guerois

Supplementary Information

Method S1 : list of the 25 gi identifiers of the sequences in the alignment

Method S2 : Details of the iterative HMM-HMM comparison protocol

Figure S1 : Evaluation graph of the models of Nbs1 and Xrs2 tandem BRCT with respect to their sequence obtained using Verify3D.

Method S3 : Detailed protocol of the simulation of molecular dynamics.

Figure S2 : simulation of molecular dynamics of the tandem BRCT of Nbs1. (A) Ca rmsd versus time (B) Secondary structures detected by DSSP versus time.

Method S4 : Detailed protocol of the simulation of docking.

Figure S3 : Distribution of the distance in the output of the HADDOCK run between the two pSer and pThr binding sites.

Figure S4 : Prediction of structural disorder in Nbs1 in the region involved in the Mdm2 binding using DISOPRED.

Bibliography .